Biochem/physiol Actions

Cell permeable: no

Target IC₅₀: 300 µM against trypsin-like serine proteases, papain and some cysteine proteases; Effective concentration: 1-100 µM

Reversible: yes

Product does not compete with ATP.

Primary Targettrypsin-like serine proteases, papain, and some cysteine proteases

General description

Peptidyl arginine aldehyde protease inhibitor produced by actinomycetes. Inhibitor of Ca²⁺-dependent endopeptidases. Has specificity similar to Leupeptin (Cat. No. 108975). Inhibits trypsin-like serine proteases, papain and some cysteine proteases (IC₅₀ = 300 µM).

Effective concentration: 1-100 µM.

A reversible protease inhibitor produced by actinomycetes. Inhibits cathepsin A and B as well as papain, and trypsin-like serine proteases. Acts by forming a hemiacetal adduct between the aldehyde group of the inhibitor and the active proteinase. Effective at concentrations range from 1 to 100 µM.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Other Notes

Lyupina, Y.V., et al. 1996. Eur. J. Pharmacol. 304, 23.Ichikawa, S., et al. 1985. J. Reprod. Fertil.73, 385Umezawa, H. 1976. Methods Enzymol.55, 678.Suda, H., et al. 1972. J. Antibiot. (Tokyo) 25, 263.

Packaging

10 mg in Plastic ampoule

Reconstitution

Following reconstitution, aliquot and freeze (-20°C) for long term storage or refrigerate (4°C) for short-term storage. Stock solutions are stable for up to 1 week at 4°C or for up to 1 month at -20°C.

Warning

Toxicity: Standard Handling (A)